MersV1, Main, Exploration, bibRecord, 002D31

Efficient identification of DNA hybridization partners in a sequence database.

Identifieur interne : 002D31 ( Main/Exploration ); précédent : 002D30; suivant : 002D32

Efficient identification of DNA hybridization partners in a sequence database.

Auteurs : Tobias P. Mann [États-Unis] ; William Stafford Noble

Source :

Bioinformatics (Oxford, England) [ 1367-4811 ] ; 2006.

RBID : pubmed:16873493

Descripteurs français

KwdFr :
- ADN (génétique), Algorithmes, Alignement de séquences (), Amorces ADN (génétique), Analyse de séquence d'ADN (), Appariement de bases (génétique), Bases de données génétiques, Données de séquences moléculaires, Hybridation in situ (), Sites de fixation, Séquence nucléotidique.
MESH :
- génétique : ADN, Amorces ADN, Appariement de bases.
- Algorithmes, Alignement de séquences, Analyse de séquence d'ADN, Bases de données génétiques, Données de séquences moléculaires, Hybridation in situ, Sites de fixation, Séquence nucléotidique.

English descriptors

KwdEn :
- Algorithms, Base Pairing (genetics), Base Sequence, Binding Sites, DNA (genetics), DNA Primers (genetics), Databases, Genetic, In Situ Hybridization (methods), Molecular Sequence Data, Sequence Alignment (methods), Sequence Analysis, DNA (methods).
MESH :
- chemical , genetics : DNA, DNA Primers.
- genetics : Base Pairing.
- methods : In Situ Hybridization, Sequence Alignment, Sequence Analysis, DNA.
- Algorithms, Base Sequence, Binding Sites, Databases, Genetic, Molecular Sequence Data.

Abstract

The specific hybridization of complementary DNA molecules underlies many widely used molecular biology assays, including the polymerase chain reaction and various types of microarray analysis. In order for such an assay to work well, the primer or probe must bind to its intended target, without also binding to additional sequences in the reaction mixture. For any given probe or primer, potential non-specific binding partners can be identified using state-of-the-art models of DNA binding stability. Unfortunately, these models rely on dynamic programming algorithms that are too slow to apply on a genomic scale.

DOI: 10.1093/bioinformatics/btl240
PubMed: 16873493

Affiliations:

Le document en format XML

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<series><title level="j">Bioinformatics (Oxford, England)</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Algorithms</term>
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<term>DNA Primers (genetics)</term>
<term>Databases, Genetic</term>
<term>In Situ Hybridization (methods)</term>
<term>Molecular Sequence Data</term>
<term>Sequence Alignment (methods)</term>
<term>Sequence Analysis, DNA (methods)</term>
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<term>Algorithmes</term>
<term>Alignement de séquences ()</term>
<term>Amorces ADN (génétique)</term>
<term>Analyse de séquence d'ADN ()</term>
<term>Appariement de bases (génétique)</term>
<term>Bases de données génétiques</term>
<term>Données de séquences moléculaires</term>
<term>Hybridation in situ ()</term>
<term>Sites de fixation</term>
<term>Séquence nucléotidique</term>
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<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA</term>
<term>DNA Primers</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Base Pairing</term>
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<term>Amorces ADN</term>
<term>Appariement de bases</term>
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<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>In Situ Hybridization</term>
<term>Sequence Alignment</term>
<term>Sequence Analysis, DNA</term>
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<term>Base Sequence</term>
<term>Binding Sites</term>
<term>Databases, Genetic</term>
<term>Molecular Sequence Data</term>
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<keywords scheme="MESH" xml:lang="fr"><term>Algorithmes</term>
<term>Alignement de séquences</term>
<term>Analyse de séquence d'ADN</term>
<term>Bases de données génétiques</term>
<term>Données de séquences moléculaires</term>
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<term>Sites de fixation</term>
<term>Séquence nucléotidique</term>
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<front><div type="abstract" xml:lang="en">The specific hybridization of complementary DNA molecules underlies many widely used molecular biology assays, including the polymerase chain reaction and various types of microarray analysis. In order for such an assay to work well, the primer or probe must bind to its intended target, without also binding to additional sequences in the reaction mixture. For any given probe or primer, potential non-specific binding partners can be identified using state-of-the-art models of DNA binding stability. Unfortunately, these models rely on dynamic programming algorithms that are too slow to apply on a genomic scale.</div>
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<country name="États-Unis"><region name="Washington (État)"><name sortKey="Mann, Tobias P" sort="Mann, Tobias P" uniqKey="Mann T" first="Tobias P" last="Mann">Tobias P. Mann</name>
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Serveur d'exploration MERS

Efficient identification of DNA hybridization partners in a sequence database.

Efficient identification of DNA hybridization partners in a sequence database.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration MERS
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